5-choice serial reaction time task

Evaluation of attention and impulsivity

5-choice-serial-reaction-time-taskTasks of attention and impulse control in humans have excellent rodent counterparts. In particular, the 5-choice serial reaction time task (5CSRTT), which is the most widely used task measuring impulsive action and attention performance, has been instrumental in defining the underlying neuroanatomy, neurochemistry and genes pertaining to these cognitive functions.

In the 5CSRTT, mice are required to respond to a brief light stimulus in one of five response apertures. The number of premature responses before the stimulus light switches on is a measure of impulsivity. Omissions of responding, accuracy of responding and the intra-individual variability in response latencies are measures of attention.

Schizophrenia-associated gene affects 5CSRTT

The Nrg3 gene is genetically associated with schizophrenia in humans, and post-mortem analysis showed an increase of Nrg3 expression in the mPFC of schizophrenia patients. In collaboration with our partners, we previously published the behavioural consequences of Nrg3 overexpression in the mPFC of mice1. Nrg3 overexpression resulted in increased impulsivity in the 5CSRTT, in line with observations of increased impulsivity in schizophrenia patients.

Sylics’ researchers and technicians have contributed their 5CSRTT expertise to numerous studies employing this task2-9.

1Neuregulin-3 in the mouse medial prefrontal cortex regulates impulsive action. Loos et al., Biol Psychiatry. 2014; 76:648
2Hyperactivity, perseveration and increased responding during attentional rule acquisition in the Fragile X mouse model. Kramvis, Loos et al., Front Behav Neurosci. 2013; 7:172
3Enhanced alcohol self-administration and reinstatement in a highly impulsive, inattentive recombinant inbred mouse strain. Loos et al., Front Behav Neurosci. 2013; 7:151
4Independent genetic loci for sensorimotor gating and attentional performance in BXD recombinant inbred strains. Loos et al., Genes Brain Behav. 2012; 11:147
5Lasting synaptic changes underlie attention deficits caused by nicotine exposure during adolescence. Counotte, Loos et al., Nat Neurosci. 2011; 14:417
6Nicotinic acetylcholine receptor β2 subunits in the medial prefrontal cortex control attention. Guillem, Loos et al., Science. 2011; 333:888
7Inhibitory control and response latency differences between C57BL/6J and DBA/2J mice in a Go/No-Go and 5-choice serial reaction time task and strain-specific responsivity to amphetamine. Loos et al., Behav Brain Res. 2010; 214:216
8Activity and impulsive action are controlled by different genetic and environmental factors. Loos et al., Genes Brain Behav. 2009; 8:817
9Strain specificity and cholinergic modulation of visuospatial attention in three inbred mouse strains. Pattij, Loos et al., Genes Brain Behav. 2007; 6:579