Rotarod

rotarodEvaluation of motor function

Motor performance and motor learning can be assessed by placing mice on a rotating rod and increasing the rotating speed. Motor performance is measured by the maximal RPM (rounds per minute) at which mice are able to keep up with the rotating rod. Motor learning can be assessed by training mice on the rod for several trials.

NMJ dysfunction affects Rotarod performance

The neuromuscular junction (NMJ) connects efferent nerves of the peripheral nervous system to muscle cells. In collaboration with our partners, we previously reported that Tomosyn-2 null mice display impaired motor performance on the rotarod without a deficit in motor learning, in line with the essential role of Tomosyn-2 in neurotransmission at the neuromuscular junction1.

A more detailed protocol for the rotarod can be found here.

1Tomosyn-2 is required for normal motor performance in mice and sustains neurotransmission at motor endplates. Geerts, Koopmans, Loos et al., Brain Struct Funct. 2014; Epub Apr 18