Three chamber sociability test

three-chamber-sociability-testEvaluation of social approach behaviour

Alterations in social behaviour are symptoms of several neuropsychiatric and neurological diseases. The three‐chamber test is a commonly used method to measure social approach behaviour in mice. After adaptation to the thee-chamber arena, a mouse is released into the middle chamber and allowed to explore the other compartments. In the adjacent ‘mouse’ compartment a docile stimulus mouse is situated in a mesh-wire container, while in the other adjacent compartment a similar container is located without stimulus mouse (object compartment). The tendency to approach or avoid the compartment with the stimulus mouse provides a measure of sociability.

Mice prefer social interaction

Wild-type mice have a tendency to socialize; they spend more time in the mouse compartment in comparison to the object compartment.