Sylics offers a range of services related to wireless recording of neuronal activity with EEG in freely moving mice.
Wireless EEG recordings in freely moving mice
Electroencephalography (EEG) is an electrophysiological method to record neuronal activity from neurons through the scalp, which is useful in the detection of, for example, sleep stages and seizure activity in humans. Similarly, by implanting electrodes superficially on the cortex of mice, intracranial EEG (a.k.a. electrocorticography, ECoG) is performed in freely moving mice to record directly from the brain. Sylics has previously detected increased epileptic spike wave discharges in freely moving Stxbp1+/- mice with wireless telemetric EEG implants. These could partially be suppressed by the anti-epileptic drug levetiracetam (Kovacevic et al 2018). Please contact us for more information.
Spike wave discharges in the Stxbp1 +/- mice coincide with twitches and jumps that are visible to a trained observer. These minute movements can accurately be tracked automatically by measure electromyography (EMG), in the neck muscle. By combining EEG with EMG, a more accurate description of seizure-like phenotypes and putative drug responses is generated.
EEG and EMG data analysis
EEG recordings contain a wealth of information at a high temporal resolution about neuronal activity in mouse models. Sylics uses commercially available software as well as a proprietary reference database and custom made software to analyze EEG recordings and detect relevant features, such as spike wave discharges. Besides EEG recordings of in house experiments, our team can also analyze EEG recordings collected by our clients. Please send us an example file of your EEG recordings, and we can have a look whether we can analyze your files.
Kovacevic J, Maroteaux G, Schut D, Loos M, Dubey M, Pitsch J, Remmelink E, Koopmans B, Crowley J, Cornelisse LN, Sullivan PF, Schoch S, Toonen RF, Stiedl O, Verhage M. Protein instability, haploinsufficiency, and cortical hyper-excitability underlie STXBP1 encephalopathy. Brain. 2018 May 1;141(5):1350-1374. doi: 10.1093/brain/awy046. PMID: 29538625; PMCID: PMC5917748. https://pubmed.ncbi.nlm.nih.gov/29538625/
Keywords: STXBP1, Munc-18, EEG
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