Circadian rhythm disruption

Jet lag protocol in running wheel actogramJet lag protocol

When housed in a cage that is equipped with a running wheel (blue cylinder in the figure), mice will voluntarily start running in it. After approximately 5 – 10 days, this voluntary wheel running behavior will stabilize and show a typical dark/light activity pattern in healthy mice.

In Sylics’ setup, rotations are detected with a magnetic system and transmitted to a PC and collected at high temporal resolution. Data is subsequently analyzed with Sylics’ proprietary AHCODA software to generate parameters of activity and circadian rhythm as displayed in the figure.

In the so-called ‘jet lag’ protocol, the dark/light cycle of the housing room is changed to experimentally disrupt the circadian rhythm. In the figure, a disruption of 6 hours was induced. It takes mice up to 5 – 10 days to fully entrain to the new cycle.

Pharmacological studies

This experimentally induced circadian rhythm disruption protocol is useful to study pharmacological effects on the recovery from jet lag and stabilization of circadian rhythm disruptions.