Cell-type specific isolation
Sylics offers a range of services related to cell-type specific isolation for downstream proteomic or genomic analysis.
Fluorescent activated cell sorting (FACS)
FACS is a flow cytometry technique that uses fluorescent markers to sort cells in suspension. Cell sorting for downstream proteomic or RNA-Seq analysis can be desirable since the brain consists of many cell types, such as neurons, astrocytes, oligodendrocytes, microglia, and neurovascular cells (epithelial cells, pericytes, smooth muscle cells, perivascular macrophages). Cells can also be sorted with FACS based on other markers for which antibodies are available, such as the presence of intracellular pathology. Through our collaboration with expert FACS laboratories we offer these services. For more information please contact us.
Laser capture microdissection (LCM)
Laser scanning microdissection (LCM) is a technique that allows isolation isolating specific pieces of brain tissue, such as those affected by tumors, protein aggregates, or vascular pathology. Brain slices can be stained with markers of interest and can then be processed with LCM to enrich for the region of interest. This therefore allows for immunofluorescence or immunohistochemical staining, followed by proteomic or genomic analysis. Through our collaboration with expert neuro-proteomics laboratories we offer these services. For more information please contact us.
More information
Please reach out to us if you want to learn more about our Cell-type specific isolation solutions. We will contact you within 1 business day.
More information
Please reach out to us if you want to learn more about our Cell-type specific isolation solutions. We will contact you within 1 business day.