Sylics offers a range of services related to detecting and quantifying DNA and RNA in the brain, CSF, and blood.
Polymerase chain reaction (PCR) is a technique to detect DNA in samples by means of amplification, allowing one to study the DNA of interest in detail. The classical gel electrophoresis-based PCR test also allows for separation of amplified sequences based on molecular weight, which is often used for genotyping of mice. PCR-based methods are also used for quantification and sequencing of DNA or RNA. For more information please contact us.
Real time qPCR
Real-time quantitative polymerase chain reaction (RT-qPCR) is still the golden standard for quantification of DNA and RNA in tissue samples. Aberrant expression of genes, such as during cellular stress or neuroinflammation, is a feature of virtually all diseases. We have used RT-qPCR to confirm viral vector mediated overexpression of target genes and modification of gene expression in disease models. For more information please contact us.
Next generation sequencing of DNA and RNA (a.k.a. RNA-Seq) are methods to detect genomic variation and gene expression, respectively. RNA-seq in particular is a popular method to determine the transcriptome of bulk tissue or individual brain cells. Through our collaboration with expert sequencing laboratories we offer these services. Determining the transcriptome of specific cell-types can be done by cell sorting before RNA-Seq (link Cell-type specific isolation) or computationally from bulk single cell data. For more information please contact us.
Please reach out to us if you want to learn more about our Genomics solutions. We will contact you within 1 business day.