Proteomics
Sylics offers a range of services related to detecting and quantifying proteins in the brain, CSF, and blood.
Western blot
Western blotting (WB) (a.k.a. immunoblotting) is a widely used technique to detect a specific protein in tissue homogenate (e.g. a brain region) or extract (e.g. insoluble proteins). WB visualizes proteins using antibody-based detection in combination with separation based on molecular weight. Although it is often faster and more reliable to quantify proteins with ELISA or SIMOA/MSD, WB therefore still offers unique information (i.e. molecular weight) compared to these plate-based techniques. Sylics offers WB services on brain tissue sections, including related techniques such as dot blot analysis. For more information please contact us.
ELISA
Enzyme-linked immunosorbent assay (ELISA) is a 96 well plate- and antibody-based technique for the quantification of proteins in tissue samples. Sylics offers a wide-range ELISA services on tissue homogenates or extracts (e.g. brain regions). This includes several formats, such as direct, sandwich, competitive, reverse ELISA, or other similar to measure complement proteins in the blood (CH50 assay). Make sure to also check out our ultra-sensitive ELISA-like protein detection systems. For more information please contact us.
SIMOA/MSD
The recent years have seen a dramatic boost in the innovation of ultra-sensitive ELISA-like protein detection systems on systems like SIMOA or MSD. This allows for sensitive and rapid quantification of low levels of proteins in brain, cerebrospinal fluid (CSF), and blood (e.g. plasma) samples. Through our collaboration with expert laboratories in the field of biomarker analyses in blood and CSF, Sylics can offer these techniques to measure minute quantities of proteins in the blood (e.g. amyloid beta, tau, NFL). For instance, amyloid beta was dramatically reduced in mice treated with a drug that lowers the production of this protein (BACE inhibitor). For more information please contact us.
Mass spectrometry
Mass spectrometry (MS) the technique of choice when the objective of the study requires simultaneous detection of a wider range of proteins in tissue samples. MS is a molecular mass-based technique with high specificity and accuracy. MS can simultaneously detect and quantify multiple proteins in a sample and is therefore suitable for more complex proteomic analysis of tissue samples. Through our collaboration with expert laboratories in the field neuro-proteomics, Sylics has gained extensive experience with MS-based proteomic analysis on mouse tissue samples, in particular on the synaptic membrane fractions. For more information please contact us.
More information
Please reach out to us if you want to learn more about our Proteomics solutions. We will contact you within 1 business day.